Part:BBa_K4786002:Design

snoopTag attached GmPilA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

1)Choice of tag sequence.The length of tag can influence the folding or assembly of GmPilA monomers.So we had to choose a glue peptide pair that is short enough.SnoopTag and SnoopCatcher is a suitable pair. 2)Choice of position to merge SnoopTag:GmPilA monomers need to fold and assemble properly and a merged peptide may influence this process.After reading relevant articles, we found that the C-terminus of GmPilA monomer is projected out from the pilus surface and previous researches found that a short peptide merged to its C-terminus would not significantly interfere with this process.

Source

GmPilA sequence comes from grnomic sequence of Geobacter metallireducens. SnoopTag comes from article: Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Programmable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1202-7.

References

[1]Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Programmable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1202-7.